解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of gamma-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred ta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-0003-x
更新日期:2006-04-01 00:00:00
abstract::Centaurea calcitrapa suspension cultures were grown either in Erlenmeyer flasks or in a mechanically stirred bioreactor. Its rheological behaviour, when fitted to the Oswald-de Waele model (power law), showed pseudoplastic characteristics in both cases. The flow behaviour index (n) decreased over the course of a growt...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-6181-0
更新日期:2006-03-01 00:00:00
abstract::A novel cDNA for the flavonoid-3',5'-hydroxylase (F35H) gene was cloned from petals of Phalaenopsis, and designated as Phf35h (accession number DQ148458 in GenBank/EMBL/DDBJ). The genomic clone of Phf35h was isolated by a PCR-based strategy. Nucleotide sequence analysis revealed that its genomic clone contains one int...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5718-6
更新日期:2006-03-01 00:00:00
abstract::Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5327-4
更新日期:2006-02-01 00:00:00
abstract::Three chloroplast genes, chlL, chlN and chlB, encoding the light-independent protochlorophyllide oxidoreductase (LIPOR) in Chlorella protothecoides CS-41 growing either photoautotrophically, mixotrophically or heterotrophically, were all transcribed constitutively independent of illumination and presence of glucose. S...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5528-x
更新日期:2006-02-01 00:00:00
abstract::Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 degrees C (assayed over 10 min). Activity at 0 degree C was nearly 35% of th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-4951-3
更新日期:2006-01-01 00:00:00
abstract::A new aqueous two phase system comprising polyethylene glycol and xanthan is reported together with its phase diagram. The phase composition of the bottom phase did not vary (PEG 1.6-1.8% w/w; xanthan 0.24-0.28% w/w) while that of the top phase varied significantly (PEG 4-5% w/w, xanthan 0.05-1.37% w/w). Unlike conven...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-4682-5
更新日期:2006-01-01 00:00:00
abstract::Quercetin was methylated with two O-methyltransferases (OMTs) expressed in E. coli. A construct (RSOMT) was designed to express two OMTs: ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group. Both OMT genes were driven by T7 promoters and had...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3893-0
更新日期:2005-12-01 00:00:00
abstract::Ochrobactrum anthropi cleaved the delta-(D-alpha-aminoadipoyl)-side chain from delta-(D-alpha-aminoadipoyl)-7-amino-4-methylcoumarin, a beta-lactamase-resistant cephalosporin C analogue. In whole cell conversions up to 1 nkat g(-1) dry cell wt were achieved. O. anthropi possesses also gamma-D-glutamyltranspeptidase ac...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3903-2
更新日期:2005-12-01 00:00:00
abstract::Although resistance of microorganisms to Hg(II) salts has been widely investigated and resistant strains have been reported from many eubacterial genera, there are few reports of mercuric ion resistance in extremophilic microorganisms. Moderately thermophilic mercury resistant bacteria were selected by growth at 62 de...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2723-8
更新日期:2005-11-01 00:00:00
abstract::A dicistronic expression vector was constructed for Chinese hamster ovary (CHO) cells that produce both selectable marker-DHFR (dihydrofolate reductase) gene and recombinant antibody cDNA from a single primary transcript via differential splicing. The vector was derived from a pDHL vector and contained the human const...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2736-3
更新日期:2005-11-01 00:00:00
abstract::Our current knowledge of protein unfolding is overwhelmingly related to reversible denaturation. However, to engineer thermostable enzymes for industrial applications and medical diagnostics, it is necessary to consider irreversible denaturation processes and/or the entire quaternary structure. In this study we have u...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3555-2
更新日期:2005-11-01 00:00:00
abstract::An extracellular chitinase from Streptomyces halstedii AJ-7, a broad spectrum antifungal biocontrol agent, was characterized and purified. The apparent molecular weight of the purified protein was 55 kDa, Km value and Vmax of the protein for colloidal chitin were 3.2 mg ml(-1) and 118 micromol h(-1), respectively. The...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-1315-y
更新日期:2005-10-01 00:00:00
abstract::A gene, rBmK Cta, encoding a chlorotoxin-like peptide from the scorpion, Buthus martensii Karsch, was synthesized according to the sequence optimized for codon usage in Escherichia coli and was expressed in E. coli BL21 (DE3) using a pExSecI expression system in which the IgG-binding domain-ZZ of protein A is fused to...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2514-2
更新日期:2005-10-01 00:00:00
abstract::Mucor circinelloides and Mortierella isabellina hydroxylated dehydroabietic acid (DehA). DehA was converted regio- and stereo-selectively by whole cells of Mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mM from 14.7 mM DehA) after 72 h in the cultivation medium contain...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3224-5
更新日期:2005-09-01 00:00:00
abstract::C3H/HeJ mice were sensitized with ovalbumin (OVA) and choleratoxin (CT) for 5 weeks, and then Bifidobacterium bifidum BGN4 was administered continuously for 7 weeks, starting 2 weeks before (pre-treatment group) and 2 weeks after (post-treatment group) the initial sensitization. After sensitization, the OVA-induced (s...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3682-9
更新日期:2005-09-01 00:00:00
abstract::Fluctuating light intensity had a more significant impact on growth of gametophytes of transgenic Laminaria japonica in a 2500 ml bubble-column bioreactor than constant light intensity. A fluctuating light intensity between 10 and 110 microE m(-2) s(-1), with a photoperiod of 14 h:10 h light:dark, was the best regime ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-0938-3
更新日期:2005-09-01 00:00:00
abstract::Three biological activities of 12 soy isoflavones were investigated in terms of the structure-activity relationship. Seven molecular descriptors and one absorption predictor were significantly related to the inhibition of cytochrome P4501 activity in HepG2 cells by soy isoflavones, yielding water solubility [correlati...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8457-9
更新日期:2005-08-01 00:00:00
abstract::Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8655-5
更新日期:2005-08-01 00:00:00
abstract::Heterologous expression in Escherichia coli often leads to production of the expressed proteins as insoluble and inactive inclusion bodies. The general strategy for protein recovery includes isolation and washing of inclusion bodies, solubilization of aggregated protein and refolding of solubilized protein. The proces...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-0024-x
更新日期:2005-08-01 00:00:00
abstract::Four Cr(VI)-reducing bacterial strains (Ochrobactrum intermedium, CrT-2, CrT-3 and CrT-4) previously isolated from chromium-contaminated sites were inoculated on to seeds of sunflower (Helianthus annuus var SF-187), which were germinated and grown along with non-inoculated controls with chromate salts (300 microg CrCl...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-7188-2
更新日期:2005-07-01 00:00:00
abstract::N(omega)-2,2,4,6,7-Pentamethyldihydrobenzofuran-5-sulfonyl (N(omega)-Pbf)-protected oligoarginine was directly conjugated to poly(amino acid) derivatives modified with a long alkyl chain. The final concentration of conjugated peptides was easily controlled by the feed ratio of oligoarginine to polymer backbone and a f...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-7838-4
更新日期:2005-07-01 00:00:00
abstract::The baculovirus expression vector system (BEVS) is used extensively for the production of proteins from exogenous cDNAs. However, BEVS is not ideal for pharmaceutical production of glycoproteins owing to the properties of the N-glycans in the expressed products and that insect cells lack several of the enzymes require...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8107-2
更新日期:2005-07-01 00:00:00
abstract::Cell adhesion to extracellular matrices, including fibronectin, results in clustering of integrins in focal adhesions. To promote the clustering of fibronectin and thus enhance its activity at the sites of focal adhesion formation, we have engineered a fusion protein containing recombinant fibronectin fragment (hFN) c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-6101-3
更新日期:2005-06-01 00:00:00
abstract::Expression of the desulfurization genes (dsz) in Mycobacterium sp. G3 is repressed by sulfate, which is the product of biodesulfurization. An expression clone, pSMTABC, was constructed by placing the dsz genes downstream of the hsp60 promoter and the constructed plasmid was electroporated into G3. The recombinant stra...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-6721-7
更新日期:2005-06-01 00:00:00
abstract::A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5794-7
更新日期:2005-06-01 00:00:00
abstract::Dibutyl phthalate (DBP) was more efficiently degraded by cutinase compared to yeast esterase; i.e. almost 80% of initial DBP (500 mg l(-1)) was decomposed within 7.5 h, and nearly 50% of the degraded DBP disappeared within the initial 30 min. The toxicity of the final DBP degradation products were investigated using v...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3631-7
更新日期:2005-05-01 00:00:00
abstract::Lipase-catalyzed synthesis of potential multifunctional ribavirin derivatives was performed in acetone. Divinyl dicarboxylates with different chain lengths (C4, C6, C9, C10) were used as acyl donors and the reactions were catalyzed by lipase immobilized on acrylic resin from Candida antarctica (CAL-B). Ribavirin was r...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5188-x
更新日期:2005-05-01 00:00:00
abstract::The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB), poly(ethylene succinate) (PES), poly(ethylene adipate) (PEA), poly(butylene su...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2217-8
更新日期:2005-04-01 00:00:00
abstract::Bacterial resistance to the aminoglycoside antibiotics is manifested primarily by enzymic modification of these drugs. One important mechanism of streptomycin modification is through ATP-dependent O-adenylation, catalyzed by streptomycin adenylyltransferase. Initial velocity patterns deduced from steady state kinetics...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2544-9
更新日期:2005-04-01 00:00:00
abstract::Tributyl phosphate (TBP) is widely used in nuclear fuel processing and other waste generating chemical industries. Although TBP is bacteriostatic, some microbes are resistant to it and may degrade it. Under dark aerobiosis, purple non-sulfur photosynthetic bacteria degraded up to 0.6 mM TBP, initially present at 2 mM,...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2882-7
更新日期:2005-04-01 00:00:00
abstract::Gluconobacter oxydans LMG 1489 was selected as the best strain for NAD(P)-dependent polyol dehydrogenase production. The highest enzyme activities were obtained when this strain was cultivated on a medium consisting of 30 g glycerol l(-1), 7.2 g peptone l(-1) and 1.8 g yeast extract l(-1). Two D-fructose reducing, NAD...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-0684-6
更新日期:2005-03-01 00:00:00
abstract::Flavonoids have been proposed to play diverse roles in plant growth and development, including defense, symbiosis, pollen development and male fertility, polar auxin transport, and protection against ultraviolet radiation. Recently, a new role in cell cycle regulation has emerged. Genetic alteration of glucuronide met...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-005-1521-7
更新日期:2005-03-01 00:00:00
abstract::A gene cassette, p35S-CNO, was designed to express three gene products driven by a single constitutive CaMV 35S promoter. The individual coding regions were linked in frame to produce a single polyprotein, using spacer sequences encoding a specific heptapeptide cleavage recognition site (ENLYFQS) for the nuclear-inclu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-1884-9
更新日期:2005-03-01 00:00:00
abstract::The culture broth of Streptomyces halstedii AJ-7 suppressed the growth of Phytophthora capsici, which causes phytophthora blight in red-peppers, with less than 1% survival of the pathogen after 12 h of treatment. The low molecular fraction (< or = 10 kDa) of the culture broth retained anti-fungal activity against P. c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-7879-0
更新日期:2005-02-01 00:00:00
abstract::The inhibition of protein-tyrosine phosphatase 1B (PTP1B) is a potential target for treatment of type 2 diabetes. Vanadium and zinc metal coordinated complexes have insulin-enhancing activities, and while vanadium compounds inhibit PTP1B, little is known on the mode of action of zinc compounds. In this study we develo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-7855-8
更新日期:2005-02-01 00:00:00
abstract::A strain of Pseudomonas stutzeri ZWLR2-1 was isolated from soil contaminated with chloronitrobenzenes and identified by 16S rDNA sequencing. This bacterium released chloride and nitrite into the medium when grown on 0.5 mM 2-chloronitrobenzene. PCR amplification and DNA sequencing revealed a DNA fragment encoding a po...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-8293-3
更新日期:2005-02-01 00:00:00
abstract::Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-co-glycolic acid, 65:35) (PLGA) films coated with poly-D: -lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for bo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6585-2
更新日期:2005-01-01 00:00:00
abstract::Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75+/-0.41 and 6.4+/-0.31 mg l-1. Production of jaceosidin and hispidulin correlated with ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6934-1
更新日期:2005-01-01 00:00:00
abstract::Chloroperoxidase from Caldariomyces fumago catalyses the oxidation of 1,2-dihydronaphthalene to (1R,2R)-(+)-dihydroxytetrahydronaphthalene in homogenous citrate buffer/ionic liquid mixtures, using t-butyl hydroperoxide as O2 donor. It tolerates up to 30 (v/v) 1,3-dimethylimidazolium methylsulfate or 1-butyl-3-methylim...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-5087-6
更新日期:2004-12-01 00:00:00